The extensive uses of organophosphates and pyrethroids have made it necessary to investigate the neurotoxicity of their combination as they may implicate in the neurodegenerative syndromes. Monoamine oxidase-A (MAO-A) and acetylcholinesterase (AChE) gene expression in the rat brain were evaluated after independent and combined intoxications with chlorpyrifos and cypermethrin. Twenty-four mature male rats were equally distributed into four groups. The first one was kept as a control group, whereas the second, third and fourth were orally gavage with chlorpyrifos (16.324 mg/kg), cypermethrin (25.089 mg/kg) and their combination (9.254 mg/kg), respectively, for 4 weeks. As compared to the control group, intoxications with chlorpyrifos and/or cypermethrin revealed significant (P < 0.05) declines in the levels of brain neurotransmitters (dopamine and serotonin) plus the enzymatic activities of MAO-A, AChE and sodium-potassium adenosine triphosphatase. The mRNA genes expression of MAO-A and AChE have also confirmed the enzymatic actions. Moreover, the oxidative injury recorded as the levels of malondialdehyde and nitric oxide markedly increased (P < 0.01), while the total thiol content reduced and the histopathological outcomes have confirmed these impacts. In conclusion, chlorpyrifos and cypermethrin revealed antagonistic inhibitions on the brain MAO-A and AChE gene regulation through neurotransmission deteriorations and oxidative damage, which could describe their contributions in the neuropathological progressions.
Keywords: Acetylcholinesterase; Chlorpyrifos; Cypermethrin; Gene expression; Monoamine oxidase.