Squid Ink Polysaccharides Protect Human Fibroblast Against Oxidative Stress by Regulating NADPH Oxidase and Connexin43

Ying Chen; Huazhong Liu; Hao Huang; Yuetang Ma; Ruihua Wang; Yong Hu; Xiufen Zheng; Chunmei Chen; Hongfeng Tang

doi:10.3389/fphar.2019.01574

Squid Ink Polysaccharides Protect Human Fibroblast Against Oxidative Stress by Regulating NADPH Oxidase and Connexin43

Front Pharmacol. 2020 Jan 17:10:1574. doi: 10.3389/fphar.2019.01574. eCollection 2019.

Authors

Ying Chen^{1

2}, Huazhong Liu³, Hao Huang², Yuetang Ma², Ruihua Wang², Yong Hu², Xiufen Zheng², Chunmei Chen², Hongfeng Tang²

Affiliations

¹ Department of Dermatology, Nanchong Central Hospital, the Second Clinical Medical College of North Sichuan Medical College, Nanchong, China.
² Department of Dermatology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde Foshan), Foshan, China.
³ College of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, China.

Abstract

Oxidation injury to skin is one of the main reasons for skin aging. The aim of the present study was to explore the protective effect of squid ink polysaccharides and its mechanism of action against H₂O₂-induced dermal fibroblast damage. Our results show that squid ink polysaccharides effectively reduce the fibroblast oxidative damage mediated by the up-regulation of NADPH oxidase and Connexin43. Concurrently, squid ink polysaccharides decrease the ROS induced up-regulation of MMP1 and MMP9 to decrease MMP-mediated skin aging. Therefore, we hypothesize that squid ink polysaccharides play an antioxidant role by inhibiting the expression of NADPH oxidase and connexin43. This provides a new target for the effective clinical prevention and treatment of oxidative skin damage.

Keywords: NADPH oxidase; connexin43; fibroblasts; oxidative stress; squid ink polysaccharides.