RNA-targeting CRISPR systems from metagenomic discovery to transcriptomic engineering

Aaron A Smargon; Yilan J Shi; Gene W Yeo

doi:10.1038/s41556-019-0454-7

RNA-targeting CRISPR systems from metagenomic discovery to transcriptomic engineering

Nat Cell Biol. 2020 Feb;22(2):143-150. doi: 10.1038/s41556-019-0454-7. Epub 2020 Feb 3.

Authors

Aaron A Smargon¹, Yilan J Shi^{1

2}, Gene W Yeo^{3

4}

Affiliations

¹ Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA.
² Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, CA, USA.
³ Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA, USA. geneyeo@ucsd.edu.
⁴ Biomedical Sciences Graduate Program, University of California, San Diego, La Jolla, CA, USA. geneyeo@ucsd.edu.

Abstract

Deployment of RNA-guided DNA endonuclease CRISPR-Cas technology has led to radical advances in biology. As the functional diversity of CRISPR-Cas and parallel systems is further explored, RNA manipulation is emerging as a powerful mode of CRISPR-based engineering. In this Perspective, we chart progress in the RNA-targeting CRISPR-Cas (RCas) field and illustrate how continuing evolution in scientific discovery translates into applications for RNA biology and insights into mysteries, obstacles, and alternative technologies that lie ahead.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Animals, Genetically Modified
CRISPR-Associated Protein 9 / genetics
CRISPR-Associated Protein 9 / metabolism
CRISPR-Cas Systems*
Gene Editing / methods*
Genetic Engineering / methods*
Genetic Therapy / methods
Humans
Metagenome*
RNA, Guide, CRISPR-Cas Systems / genetics
RNA, Guide, CRISPR-Cas Systems / metabolism
Transcriptome*

Substances

RNA, Guide, CRISPR-Cas Systems
CRISPR-Associated Protein 9

Abstract

Publication types

MeSH terms

Substances

Grants and funding