(1) Background: Recent studies have investigated the expression of taste-related genes in the organs of various animals, including humans; however, data for additional taxa are needed to facilitate comparative analyses within and among species. (2) Methods: We investigated the expression of taste-related genes in the intestines of rhesus macaques, the non-human primates most commonly used in experimental models. (3) Results: Based on RNAseq and qRT-PCR, genes encoding bitter taste receptors and the G-protein gustducin were expressed in the gut of rhesus macaques. RNAscope analysis showed that one of the bitter receptors, TAS2R38, was expressed in some cells in the small intestine, and immunohistochemical analysis revealed the presence of T2R38-positive cells in the villi of the intestines. (4) Conclusions: These results suggest that bitter receptors are expressed in the gut of rhesus macaques, supporting the use of macaques as a model for studies of human taste, including gut analyses.
Keywords: RNAseq; bitter taste receptors; macaque.
Conflict of interest statement
The authors declare no conflict of interest.
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- KAKENHI 18H04005,19K21586, 16H01338, 15H02421, 15H05242, and 25257409 to HI/Japan Society for the Promotion of Science
- to HI/Kobayashi International Scholarship Foundation
- 18-III514/Terumo Foundation for Life Sciences and Arts
- to HI/Umami Manufacturers Association of Japan
- Bilateral Research Program between Japan and Indonesia, Japan and Korea to H.I./Japan Society for the Promotion of Science