Evaluation of the biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa on human umbilical cord CD146+ stem cells and stem cell-based decellularized matrix

Cell Tissue Bank. 2020 Jun;21(2):215-231. doi: 10.1007/s10561-020-09815-6. Epub 2020 Feb 4.


This study aims to evaluate the CD146+ stem cells obtained from the human umbilical cord and their extracellular matrix proteins on in vitro Pseudomonas aeruginosa and Staphylococcus aureus biofilms to understand their possible antimicrobial activity. CD146+ stem cells were determined according to cell surface markers and differentiation capacity. Characterization of the decellularized matrix was done with DAPI, Masson's Trichrome staining and proteome analysis. Cell viability/proliferation of cells in co-cultures was evaluated by WST-1 and crystal-violet staining. The effects of cells and decellularized matrix proteins on biofilms were investigated on a drip flow biofilm reactor and their effects on gene expression were determined by RT-qPCR. We observed that CD146/105+ stem cells could differentiate adipogenically and decellularized matrix showed negative DAPI and positive collagen staining with Masson' s Trichrome. Proteome analysis of the decellularized matrix revealed some matrix components and growth factors. Although the decellularized matrix significantly reduced the cell counts of P. aeruginosa, no significant difference was observed for S. aureus cells in both groups. Supporting data was obtained from the gene expression results of P. aeruginosa with the significant down-regulation of rhlR and lasR. For S. aureus, icaADBC genes were significantly up-regulated when grown on the decellularized matrix.

Keywords: Biofilm; CD146+ stem cells; Decellularized matrix; Pseudomonas aeruginosa; Staphylococcus aureus; Umbilical cord.

MeSH terms

  • Biofilms / growth & development*
  • Biomarkers / metabolism
  • CD146 Antigen / metabolism*
  • Cell Differentiation
  • Cell Proliferation
  • Cell Shape
  • Cells, Cultured
  • Coculture Techniques
  • Extracellular Matrix / metabolism*
  • Extracellular Matrix Proteins / metabolism
  • Gene Expression Regulation, Bacterial
  • Humans
  • Proteome / metabolism
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / physiology*
  • Pseudomonas aeruginosa / ultrastructure
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / physiology*
  • Staphylococcus aureus / ultrastructure
  • Stem Cells / cytology*
  • Umbilical Cord / cytology*


  • Biomarkers
  • CD146 Antigen
  • Extracellular Matrix Proteins
  • Proteome