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, 16, 153-160

Exosomal Long Non-Coding RNA Expression From Serum of Patients With Acute Minor Stroke


Exosomal Long Non-Coding RNA Expression From Serum of Patients With Acute Minor Stroke

Xiaonan Xu et al. Neuropsychiatr Dis Treat.


Background: Acute minor stroke (AMS) is one kind of hypoxic ischemic necrosis with no more than 4 National Institutes of Health Stroke Scale (NIHSS) score. However, the early diagnosis of AMS is tough for lack of effective molecular markers. Recently, many long non-coding RNAs (lncRNAs) associated with AMS have been gradually revealed. Here, we aim to find the potential biomarkers of lncRNAs in exosomes isolated from blood serum of patients with AMS for early detection.

Methods: RNA-seq technique, KEGG pathway analysis and GO enrichment analysis were used in this study. Besides, reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) was used to validate expression levels of four of eleven differentially expressed lncRNAs (lnc-CRKL-2, lnc-NTRK3-4, RPS6KA2-AS1 and lnc-CALM1-7) involved in the neurotrophin signaling pathway.

Results: The expression levels of lnc-CRKL-2 (mean value 48, standard deviation 4.583, P = 0.003) and lnc-NTRK3-4 (mean value 32.3, standard deviation 2.08, P = 0.001) were increased significantly in AMS patients, while the expression levels of RPS6KA2-AS1 (mean value -118.7, standard deviation 7.09, P = 0.001) and lnc-CALM1-7 (mean value -148.7, standard deviation 6.10, P = 0.001) were decreased dramatically.

Conclusion: In conclusion, these four new revealed lncRNAs may be used as novel joint biomarkers for the early detection of AMS.

Keywords: LncRNAs; biomarker; exosomes; stroke.

Conflict of interest statement

The authors report no conflicts of interest in this work.


Figure 1
Figure 1
Western blot results demonstrate the expression of CD63 and Tsg101 in exosomes isolated from the serum of patients with acute minor stroke and healthy controls.
Figure 2
Figure 2
Characteristics of the lncRNA expression were shown between healthy controls and patients. (A) Heatmap of differential expressed lncRNAs with a P-value <0.05 was presented. (B) Volcano plot of differential expressed lncRNAs was shown. (CF) Pathways and diseases that differentially expressed lncRNAs involved were exhibited.
Figure 3
Figure 3
Validation of the expression levels of lnc-CRKL-2, lnc-NTRK3-4, RPS6KA2-AS1, lnc-CALM1-7 in one hundred patients with AMS was performed by RT-qPCR. (A and B) The expression levels of lnc-CRKL-2 and lnc-NTRK3-4 were significantly increased in patients compared with healthy controls (Both P < 0.001). (C and D) The expression levels of RPS6KA2-AS1 and lnc-CALM1-7 were obviously decreased in patients compared with healthy controls (Both P < 0.001). **represents P < 0.001.

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