A combined Far-FTIR, FTIR Spectromicroscopy, and DFT Study of the Effect of DNA Binding on the [4Fe4S] Cluster Site in EndoIII

Sci Rep. 2020 Feb 6;10(1):1931. doi: 10.1038/s41598-020-58531-4.

Abstract

Endonuclease III (EndoIII) is a DNA glycosylase that contains the [4Fe4S] cluster, which is essential for the protein to bind to damaged DNA in a process called base excision repair (BER). Here we propose that the change in the covalency of Fe-S bonds of the [4Fe4S] cluster caused by double-stranded (ds)-DNA binding is accompanied by a change in their strength, which is due to alterations of the electronic structure of the cluster. Micro-FTIR spectroscopy in the mid-IR region and FTIR spectroscopy in the far IR (450 and 300 cm-1) were used independently to study the structural changes in EndoIII and the behavior of the [4Fe4S] cluster it contains, in the native form and upon its binding to ds-DNA. Structural changes in the DNA itself were also examined. The characteristics vibrational modes, corresponding to Fe-S (sulfide) and Fe-S (thiolate) bonds were identified in the cluster through far IR spectroscopy as well through quantum chemistry calculations. Based on the experimental results, these vibrational modes shift in their spectral positions caused by negatively charged DNA in the vicinity of the cluster. Modifications of the Fe-S bond lengths upon DNA binding, both of the Fe-S (sulfide) and Fe-S (thiolate) bonds in the [4Fe4S] cluster of EndoIII are responsible for the stabilization of the cluster towards higher oxidation state (3+), and hence its redox communication along the ds-DNA helix.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites / physiology
  • DNA / metabolism*
  • DNA Damage / physiology
  • DNA Glycosylases / metabolism
  • DNA Repair / physiology
  • DNA-Binding Proteins / metabolism*
  • Deoxyribonuclease (Pyrimidine Dimer) / metabolism*
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / metabolism*
  • Iron-Sulfur Proteins / metabolism*
  • Oxidation-Reduction
  • Spectroscopy, Fourier Transform Infrared / methods

Substances

  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • Iron-Sulfur Proteins
  • DNA
  • Deoxyribonuclease (Pyrimidine Dimer)
  • NTH protein, E coli
  • DNA Glycosylases