Characterization of the First mec A-Positive Multidrug-Resistant Staphylococcus pseudintermedius Isolated from an Argentinian Patient

Microb Drug Resist. 2020 Jul;26(7):717-721. doi: 10.1089/mdr.2019.0308. Epub 2020 Feb 7.


Staphylococcus pseudintermedius is commonly associated with colonization or infection in dogs, and was identified as a novel species within the genus Staphylococcus in 2006. Methicillin resistance emerged in S. pseudintermedius during the last decade. We describe here a genomic characterization of the first methicillin-resistant S. pseudintermedius (MRSP) recovered from a human patient in Argentina. The strain was phenotypically identified as MRSP 8510 by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and antimicrobial susceptibility testing. We assessed genetic characterization by mecA PCR, SCCmec (staphylococcal chromosomal cassette) typing, and whole-genome sequencing. MRSP 8510 was phenotypically resistant to six classes of antimicrobial agents, consistent with the genes found in its genome. We concluded that MRSP 8510 was a multidrug-resistant ST1412 isolate. This study highlights the importance of the detection and characterization of pathogens with potential risks of zoonotic transmission to humans, as they may constitute a reservoir of genes associated with antimicrobial resistance.

Keywords: MDR; MRSP; Staphylococcus pseudintermedius.

Publication types

  • Case Reports

MeSH terms

  • Aged, 80 and over
  • Anti-Bacterial Agents / pharmacology*
  • Argentina
  • Drug Resistance, Multiple, Bacterial*
  • Female
  • Humans
  • Methicillin Resistance*
  • Multilocus Sequence Typing
  • Staphylococcus / isolation & purification*
  • Whole Genome Sequencing


  • Anti-Bacterial Agents

Supplementary concepts

  • Staphylococcus pseudintermedius