Functional expression of ZNF467 and PCBP2 supports adipogenic lineage commitment in adipose-derived mesenchymal stem cells

Gene. 2020 May 5;737:144437. doi: 10.1016/j.gene.2020.144437. Epub 2020 Feb 4.

Abstract

Bone marrow-derived mesenchymal stromal/stem cells (BMSCs) have the potential to be employed in many different skeletal therapies. A major limitation to utilizing BMSCs as a therapeutic strategy in human disease and tissue regeneration is the low cell numbers obtained from initial isolation necessitating multiple cell passages that can lead to decreased cell quality. Adipose-derived mesenchymal stromal/stem cells (AMSCs) have been proposed as an alternative cell source for regenerative therapies; however the differentiation capacity of these cells differs from BMSCs. To understand the differences between BMSCs and AMSCs, we compared the global gene expression profiles of BMSCs and AMSCs and identified two genes, PCBP2 and ZNF467 that were differentially expressed between AMSCs and BMSCs. We demonstrate that PCBP2 and ZNF467 impact adipogenic but not osteogenic differentiation, further supporting evidence that AMSCs and BMSCs appear to be adapted to their microenvironment.

Keywords: Adipogenic differentiation; Adipose-derived mesenchymal stromal/stem cells.

MeSH terms

  • Adipose Tissue / cytology*
  • Cell Differentiation*
  • Cell Lineage
  • Cells, Cultured
  • Humans
  • Mesenchymal Stem Cells / cytology*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Transcription Factors, General / genetics
  • Transcription Factors, General / metabolism*

Substances

  • PCBP2 protein, human
  • RNA-Binding Proteins
  • Transcription Factors, General
  • ZNF467 protein, human