Facile synthesis of paper based graphene electrodes for point of care devices: A double stranded DNA (dsDNA) biosensor

J Mohanraj; D Durgalakshmi; R Ajay Rakkesh; S Balakumar; Saravanan Rajendran; Hassan Karimi-Maleh

doi:10.1016/j.jcis.2020.01.089

Facile synthesis of paper based graphene electrodes for point of care devices: A double stranded DNA (dsDNA) biosensor

J Colloid Interface Sci. 2020 Apr 15:566:463-472. doi: 10.1016/j.jcis.2020.01.089. Epub 2020 Jan 27.

Authors

J Mohanraj¹, D Durgalakshmi², R Ajay Rakkesh³, S Balakumar⁴, Saravanan Rajendran⁵, Hassan Karimi-Maleh⁶

Affiliations

¹ Department of Medical Physics, CEG Campus, Anna University, Chennai 600 025, India.
² Department of Medical Physics, CEG Campus, Anna University, Chennai 600 025, India. Electronic address: durgaklakshmi@gmail.com.
³ CAS in Crystallography, Guindy Campus, University of Madras, Chennai 600 025, India.
⁴ National Centre for Nanoscience and Nanotechnology, Guindy Campus, University of Madras, Chennai 600 025, India.
⁵ Faculty of Engineering, Department of Mechanical Engineering, University of Tarapaca, Avda. General Velasquez, 1775 Arica, Chile.
⁶ School of Resources and Enviroment, University of Electronic Science and Technology of China, P.O. Box 611731, Xiyuan Ave, Chengdu, PR China; Laboratory of Nanotechnology, Department of Chemical Engineering, Quchan University of Technology, Quchan 94771-67335, Iran; Department of Applied Chemistry, University of Johannesburg, Johannesburg 17011, South Africa. Electronic address: hassan@uestc.edu.cn.

PMID: 32032811
DOI: 10.1016/j.jcis.2020.01.089

Abstract

The demand for high-quality graphene for electronic applications is increasing due to its high carrier mobility and electrical conductivity. In this connection, printing technology is a reliable method towards the fabrication of conductive, disposable graphene-based electrode for low-cost sensor application. Herein, we aimed to report the synthesis of high-quality graphene nanosheets obtained by electrochemical exfoliation of biomass-derived from corn cob. The conductive ink was prepared from this exfoliated graphene and was utilized for the preparation of paper-based graphene electrode towards double stranded DNA (dsDNA) sensor application. This paper, based graphene electrode opens the possibility of direct electrochemical analysis of analyte without any sample preparation. In this study, two irreversible oxide peaks were obtained from paper-based printed graphene electrode, corresponds to oxidation of guanine (G) and adenine (A) of dsDNA in the linear range of 0.2 pg mL^-1 to 5 pg mL^-1 with the detection limit of 0.68 pg mL^-1 and the sensitivity of 0.00656 mA pg^-1 cm^-2. Further, a small-scale printable circuit is fabricated using this graphene shows good conductivity of 1.145x10³(S/m).

Keywords: Biomass; DNA; Electrochemical; Graphene; Sensor.

MeSH terms

Biosensing Techniques* / instrumentation
DNA / analysis*
Electrochemical Techniques* / instrumentation
Electrodes
Graphite / chemistry*
Humans
Paper*
Particle Size
Point-of-Care Systems*
Surface Properties

Substances

Graphite
DNA