Paracoccus alkanivorans sp. nov., isolated from a deep well with oil reservoir water

Abstract

A Gram-stain-negative, non-motile and ovoid bacterial strain, designated 4-2^T, was isolated from oil-contaminated water which was collected from Xinjiang Province, north-west PR China. The 16S rRNA gene sequence analysis showed that strain 4-2^T belonged to the genus Paracoccus. The species with highest similarity to strain 4-2^T was Paracoccus saliphilus YIM 90738^T (97.83 %), followed by 'Paracoccus siganidrum' M26 (97.83 %) and Paracoccus endophyticus SYSUP0003^T (97.25 %). The average nucleotide identity values between 4-2^T and three type strains were 84.69, 77.88 and 74.07 %, respectively. The genomic DNA G+C content of strain 4-2^T was 61.4 mol%. Chemotaxonomical characteristic results showed that the respiratory quinone was ubiquinone Q-10 and the major fatty acids were summed feature 8 (C_{18 : 1} ω7c or C_{18 : 1} ω6c) and C_{19 : 0} cyclo ω8c. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unidentified phospholipids, an unidentified aminolipid and an unidentified polar lipid. The predominant polyamines were putrescine, cadaverine and spermidine. On the basis of phenotypic, chemotaxonomic and phylogenetic inferences, strain 4-2^T represents a novel species of the genus Paracoccus, for which the name Paracoccus alkanivorans sp. nov. is proposed. The type strain is 4-2^T (=CGMCC 1.13669^T=LMG 30882^T).

Keywords: Paracoccus alkanivorans sp. nov.; average nucleotide identity; oil-contaminated water; polyphasic taxonomy.