Background: Endometriosis (EM) is a common gynecological disease in women of reproductive age. These patients in approximately 80% suffer the various degree pain. This study will investigate synergistically the mechanism of the higher-position central sensitization and offer a pre-clinical experiment evidence for treatment of various location-EM patients with pain.
Methods: Twenty Sprague-Dawley rats were induced three types EM including abdominal EM (n=5), gastrocnemius EM (n=5) and ovary EM group (n=5) and one sham control group (n=5). All groups were measured the pain sensitization by hotplate test, then scanned by the functional magnetic resonance imaging (fMRI). The resting-state fMRI (rs-fMRI) date was analyzed using regional homogeneity (ReHo) approach to find out the abnormal functional activity brain regions. Nissl staining method observed the state of neurons in aberrant ReHo signal brain regions.
Results: Rats with EM pain sensitization were increased in abdominal EM and gastrocnemius EM than ovary EM group and sham control. The ReHo value is decreased in gastrocnemius EM in right thalamus and left olfactory tubercle compared with other three groups. The number of neurons was decreased; cavitation around nucleus, and pyknotic homogenous nuclei. Nissl bodies were stained deeply, and the shape was irregular in gastrocnemius EM by Nissl staining in right thalamus. In left olfactory tubercle, there was no significant difference in 4 groups.
Conclusions: The thalamus may be the potential key brain region for the central sensitization mechanism of various location-EM pain. The oxidative activation may be weakened in thalamus in gastrocnemius EM group with more severe pain. This finding could lend support for future research on the imageology and pathology of various location-EM pain.
Keywords: Various location-endometriosis; brain functional activities; central sensitization; models; pain; regional homogeneity (ReHo); resting-state functional magnetic resonance imaging (rs-fMRI).
2019 Annals of Translational Medicine. All rights reserved.
Conflict of interest statement
Conflicts of Interest: The authors have no conflicts of interest to declare.
Central Sensitization-Related Changes in Brain Function Activity in a Rat Endometriosis-Associated Pain Model.J Pain Res. 2020 Jan 13;13:95-107. doi: 10.2147/JPR.S232313. eCollection 2020. J Pain Res. 2020. PMID: 32021399 Free PMC article.
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