Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

Pingyong Wang; Haibo Wu; Guangwei Zhao; Yuhua He; Weihu Kong; Jian Zhang; Shuimiao Liu; Mengli Liu; Keyun Hu; Lifeng Liu; Yongyang Xu; Zhihong Xu

doi:10.1371/journal.pone.0227284

Transcriptome analysis clarified genes involved in resistance to Phytophthora capsici in melon

PLoS One. 2020 Feb 12;15(2):e0227284. doi: 10.1371/journal.pone.0227284. eCollection 2020.

Authors

Pingyong Wang¹, Haibo Wu², Guangwei Zhao¹, Yuhua He¹, Weihu Kong¹, Jian Zhang¹, Shuimiao Liu¹, Mengli Liu¹, Keyun Hu¹, Lifeng Liu¹, Yongyang Xu¹, Zhihong Xu¹

Affiliations

¹ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan Province, China.
² Hainan Sanya Trial Center for Crops Breeding of Xinjiang Academy of Agricultural Sciences, Sanya, Hainan Province, China.

Abstract

Phytophthora blight caused by Phytophthora capsici is a devastating disease for melon plant. However, the underlying resistance mechanisms are still poorly understood. In this study, the transcriptome differences between the resistant ZQK9 and susceptible E31 at 0, 3, and 5 days post-inoculation (dpi) were identified by RNA-seq. A total of 1,195 and 6,595 differentially expressed genes (DEGs) were identified in ZQK9 and E31, respectively. P. capsici infection triggered massive transcript changes in the inoculated tissues. Genes related to plant defense responses were activated, which was reflected by a lot of up-regulated DEGs involved in pathogenesis-related (PR) genes, hormones biosynthesis and signal transduction, secondary metabolites biosynthesis and cell wall modification in resistant ZQK9. The dataset generated in this study may provide a basis for identifying candidate resistant genes in melon against P. capsici and lay a foundation for further research on the molecular mechanisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cucurbitaceae / genetics*
Disease Resistance / genetics*
Gene Expression Profiling / methods
Gene Expression Regulation, Plant / genetics*
Phytophthora / growth & development
Plant Diseases / genetics*
Plant Proteins / genetics*
Transcriptome / genetics*

Substances

Plant Proteins

Grants and funding

This work was supported by grants from the National Natural Science Foundation of China (Grant Numbers 31801888 to PW and 31872134 to LL), the China Agriculture Research System (Grant No. CARS-25 to YX), the Agricultural Science and Technology Innovation Program (ASTIP) (Grant No. CAAS-ASTIP-2019-ZFRI to YX), the Central Public-interest Scientific Institution Basal Research Fund (Grant No. 1610192019201 to PW) and the Modern Agricultural Industry Technology of Henan province (Grant No. S2014-11-G03 to ZX).