Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice ( Oryza sativa L.)

Int J Mol Sci. 2020 Feb 10;21(3):1155. doi: 10.3390/ijms21031155.

Abstract

High temperature at anthesis is one of the most serious stress factors for rice (Oryza sativa L.) production, causing irreversible yield losses and reduces grain quality. Illustration of thermotolerance mechanism is of great importance to accelerate rice breeding aimed at thermotolerance improvement. Here, we identified a new thermotolerant germplasm, SDWG005. Microscopical analysis found that stable anther structure of SDWG005 under stress may contribute to its thermotolerance. Dynamic transcriptomic analysis totally identified 3559 differentially expressed genes (DEGs) in SDWG005 anthers at anthesis under heat treatments, including 477, 869, 2335, and 2210 for 1, 2, 6, and 12 h, respectively; however, only 131 were regulated across all four-time-points. The DEGs were divided into nine clusters according to their expressions in these heat treatments. Further analysis indicated that some main gene categories involved in heat-response of SDWG005 anthers, such as transcription factors, nucleic acid and protein metabolisms related genes, etc. Comparison with previous studies indicates that a core gene-set may exist for thermotolerance mechanism. Expression and polymorphic analysis of agmatine-coumarin-acyltransferase gene OsACT in different accessions suggested that it may involve in SDWG005 thermotolerance. This study improves our understanding of thermotolerance mechanisms in rice anthers during anthesis, and also lays foundation for breeding thermotolerant varieties via molecular breeding.

Keywords: anther; anthesis; heat stress; rice; transcriptome.

MeSH terms

  • Acetyltransferases / genetics
  • Acetyltransferases / metabolism
  • Flowers / genetics
  • Flowers / growth & development
  • Oryza / genetics*
  • Oryza / growth & development
  • Oryza / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Thermotolerance*
  • Transcriptome*

Substances

  • Plant Proteins
  • Acetyltransferases