Abstract
Pb2+ is a xenobiotic metal ion that competes for Ca2+-binding sites in proteins. Using the peripheral Ca2+-sensing domains of Syt1, we show that the chelating pH buffer Bis-Tris enables identification and functional characterization of high-affinity Pb2+ sites that are likely to be targeted by bioavailable Pb2+.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Binding Sites
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Binding, Competitive
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Buffers
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Calcium / chemistry
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Cell Membrane / chemistry
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Hydrogen-Ion Concentration
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Lead / chemistry*
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Protein Binding
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Protein Domains
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Synaptotagmin I / chemistry*
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Xenobiotics / chemistry*
Substances
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Buffers
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Synaptotagmin I
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Xenobiotics
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Lead
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Calcium