Phage vB_BveM-Goe7 represents a new genus in the subfamily Bastillevirinae

Arch Virol. 2020 Apr;165(4):959-962. doi: 10.1007/s00705-020-04546-1. Epub 2020 Feb 12.

Abstract

Bacillus velezensis FZB42 is a Gram-positive, endospore-forming rhizobacterium that is associated with plant roots and promotes plant growth. It was used as host to isolate phage vB_BveM-Goe7 (Goe7). Goe7 exhibits a Myoviridae morphology with a contractile tail and an icosahedral head. Its genome is 158,674 bp in size and contains 5137-bp-long terminal repeats (LTRs). It also contains five tRNA-encoding genes and 251 coding DNA sequences (CDS), of which 65 were annotated. The adsorption constant of Goe7 is 6.1 ± 0.24 × 10-8 ml/min, with a latency period of 75 min and a burst size of 114 particles per burst. A BLASTn sequence comparison against the non-redundant nucleotide database of NCBI revealed that Goe7 is most similar to Bacillus subtilis phage vB_BsuM-Goe3.

MeSH terms

  • Bacillus / virology*
  • Bacteriophages / classification
  • Bacteriophages / genetics
  • Bacteriophages / isolation & purification*
  • Bacteriophages / ultrastructure
  • Genome, Viral
  • Myoviridae / classification
  • Myoviridae / genetics
  • Myoviridae / isolation & purification*
  • Myoviridae / ultrastructure
  • Open Reading Frames
  • Phylogeny

Supplementary concepts

  • Bacillus velezensis