The calcineurin β-like interacting protein kinase CIPK25 regulates potassium homeostasis under low oxygen in Arabidopsis

J Exp Bot. 2020 May 9;71(9):2678-2689. doi: 10.1093/jxb/eraa004.


Hypoxic conditions often arise from waterlogging and flooding, affecting several aspects of plant metabolism, including the uptake of nutrients. We identified a member of the CALCINEURIN β-LIKE INTERACTING PROTEIN KINASE (CIPK) family in Arabidopsis, CIPK25, which is induced in the root endodermis under low-oxygen conditions. A cipk25 mutant exhibited higher sensitivity to anoxia in conditions of potassium limitation, suggesting that this kinase is involved in the regulation of potassium uptake. Interestingly, we found that CIPK25 interacts with AKT1, the major inward rectifying potassium channel in Arabidopsis. Under anoxic conditions, cipk25 mutant seedlings were unable to maintain potassium concentrations at wild-type levels, suggesting that CIPK25 likely plays a role in modulating potassium homeostasis under low-oxygen conditions. In addition, cipk25 and akt1 mutants share similar developmental defects under waterlogging, further supporting an interplay between CIPK25 and AKT1.

Keywords: Anoxia; Arabidopsis; CIPK25; calcineurin β-like interacting protein kinase; hypoxia; potassium homeostasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins* / genetics
  • Arabidopsis Proteins* / metabolism
  • Arabidopsis* / genetics
  • Arabidopsis* / metabolism
  • Calcineurin
  • Homeostasis
  • Oxygen*
  • Plant Roots / metabolism
  • Potassium / metabolism*
  • Potassium Channels / genetics
  • Protein Serine-Threonine Kinases* / genetics
  • Protein Serine-Threonine Kinases* / metabolism


  • Arabidopsis Proteins
  • Potassium Channels
  • AT5G25110 protein, Arabidopsis
  • Protein Serine-Threonine Kinases
  • Calcineurin
  • Potassium
  • Oxygen