Cell counts in hematoxylin-stained, flat-mounted retinae revealed that the inner nuclear (INL) and cone mosaic layers (CML) of the goldfish retina contained a high density of cells along the temporal boundary between dorsal and ventral retina. Our findings indicate that the goldfish contains a region situated in the temporal retina in which the density of a wide variety of cell types, that span several retinal layers, is elevated. This study indicates that the dorsotemporal retina, which is the first retinal region to develop, contains the highest density of cells.