Amide additives improve RDC measurements in polyacrylamide

J Biomol NMR. 2020 Mar;74(2-3):119-124. doi: 10.1007/s10858-020-00305-1. Epub 2020 Feb 13.

Abstract

Residual dipolar couplings (RDCs) provide valuable NMR parameters that can be used for structural calculation and verification. Measuring RDCs requires aligning macromolecules using one of various types of alignment media. Of different alignment media options, stretched or compressed polyacrylamide gels are advantageous due to their chemical stability. However, polyacrylamide interacts with proteins and significantly broadens NMR resonances. In this study, we found that the amide-containing compounds asparagine, glutamine and propionamide improve spectral quality of proteins in polyacrylamide gel without significantly reducing the magnitude of RDC values. Moreover, we showed that propionamide is an attractive additive that increases protein solubility without interfering with protein stability, ligand binding or NMR pulse width, suggesting its potential applications for our NMR methods.

Keywords: Alignment medium; Asparagine; Glutamine; Propionamide; Protein solubility; Residual dipolar couplings.

MeSH terms

  • Acrylic Resins / chemistry*
  • Nuclear Magnetic Resonance, Biomolecular*

Substances

  • Acrylic Resins
  • polyacrylamide