Orthotopic Xenograft Mouse Model in Esophageal Squamous Cell Carcinoma

Valen Z Yu; Joseph C Y Ip; Josephine M Y Ko; Lihua Tao; Alfred K Lam; Maria L Lung

doi:10.1007/978-1-0716-0377-2_12

Orthotopic Xenograft Mouse Model in Esophageal Squamous Cell Carcinoma

Methods Mol Biol. 2020:2129:149-160. doi: 10.1007/978-1-0716-0377-2_12.

Authors

Valen Z Yu¹, Joseph C Y Ip^{2

3}, Josephine M Y Ko^{2

3}, Lihua Tao², Alfred K Lam⁴, Maria L Lung⁵

Affiliations

¹ Department of Clinical Oncology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong. zvyu@hku.hk.
² Department of Clinical Oncology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
³ Vium Inc., San Mateo, CA, USA.
⁴ Cancer Molecular Pathology, School of Medicine, Griffith University, Gold Coast, Queensland, Australia.
⁵ Department of Clinical Oncology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong. mlilung@hku.hk.

PMID: 32056176
DOI: 10.1007/978-1-0716-0377-2_12

Abstract

Orthotopic xenograft model recapitulates the faithful organ-specific microenvironment and facilitates analyses involving tumor-stromal interactions that are crucial for developing new-generation cancer therapy. Herein, we describe the detailed rationales and protocols of a versatile orthotopic xenograft model for esophageal squamous cell carcinoma.

Keywords: Bioluminescence imaging; Esophageal squamous cell carcinoma; Live animal imaging; Orthotopic model; Xenograft.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
Disease Models, Animal
Esophageal Neoplasms / pathology
Esophageal Squamous Cell Carcinoma / classification
Esophageal Squamous Cell Carcinoma / pathology*
Esophagus / pathology
Heterografts
Humans
Mice
Mice, Nude
Mouth Neoplasms / pathology
Transplantation, Heterologous
Tumor Microenvironment
Xenograft Model Antitumor Assays / methods*