Umbilical cord as an analytical matrix - A technical note

doi:10.1016/j.placenta.2019.12.001

. 2020 Jan 15:90:42-44.

doi: 10.1016/j.placenta.2019.12.001. Epub 2019 Dec 3.

Umbilical cord as an analytical matrix - A technical note

Hayley R Price¹, Camron Chehroudi², Stuart J Knight¹, Alexander D Smith¹, Dickson Lai¹, Hugh Kim², Tricia E Wright³, Michael Wh Coughtrie¹, Abby C Collier⁴

Affiliations

¹ Faculty of Pharmaceutical Sciences, The University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
² Centre for Blood Research, 2350 Health Sciences Mall, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada; Faculty of Dentistry, 2199 Wesbrook Mall, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada; Department of Biochemistry and Molecular Biology, 2350 Health Sciences Mall, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.
³ Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, CA, 94115, USA.
⁴ Faculty of Pharmaceutical Sciences, The University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada. Electronic address: abby.collier@ubc.ca.

PMID: 32056550
DOI: 10.1016/j.placenta.2019.12.001

Umbilical cord as an analytical matrix - A technical note

Hayley R Price et al. Placenta. 2020.

. 2020 Jan 15:90:42-44.

doi: 10.1016/j.placenta.2019.12.001. Epub 2019 Dec 3.

Authors

Hayley R Price¹, Camron Chehroudi², Stuart J Knight¹, Alexander D Smith¹, Dickson Lai¹, Hugh Kim², Tricia E Wright³, Michael Wh Coughtrie¹, Abby C Collier⁴

Affiliations

¹ Faculty of Pharmaceutical Sciences, The University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada.
² Centre for Blood Research, 2350 Health Sciences Mall, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada; Faculty of Dentistry, 2199 Wesbrook Mall, The University of British Columbia, Vancouver, BC, V6T 1Z3, Canada; Department of Biochemistry and Molecular Biology, 2350 Health Sciences Mall, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.
³ Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, CA, 94115, USA.
⁴ Faculty of Pharmaceutical Sciences, The University of British Columbia, 2405 Wesbrook Mall, Vancouver, BC, V6T 1Z3, Canada. Electronic address: abby.collier@ubc.ca.

PMID: 32056550
DOI: 10.1016/j.placenta.2019.12.001

Abstract

The umbilical cord (UC) connects the fetal blood supply to the placenta, so is exposed to all systemic endo- and xenobiotics. We have extensive experience using UC as an analytical matrix for detecting and/or quantitating drugs, chemicals and endogenous compounds. This technical note describes advantages (large amount available, ease of collection, small sample needed for use, rapid availability) and challenges (clinical relationships, processing difficulties, matrix effects on analytes and detection technologies) of UC as an analytical matrix in ELISA and LC/MS platforms, and provides guidance for successfully working with this tissue.

Keywords: Drug screening; ELISA; Laboratory methods; Mass spectrometry; Maternal-fetal outcomes; Pharmacokinetics; Toxicology.

PubMed Disclaimer

Conflict of interest statement

Declaration of competing interest The authors declare no conflicts of interest.

Cited by

Drug exposure during pregnancy: Current understanding and approaches to measure maternal-fetal drug exposure.
Hudson RE, Metz TD, Ward RM, McKnite AM, Enioutina EY, Sherwin CM, Watt KM, Job KM. Hudson RE, et al. Front Pharmacol. 2023 Mar 23;14:1111601. doi: 10.3389/fphar.2023.1111601. eCollection 2023. Front Pharmacol. 2023. PMID: 37033628 Free PMC article. Review.

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions

Grants and funding

LinkOut - more resources

Full Text Sources
- Elsevier Science

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Umbilical cord as an analytical matrix - A technical note

Affiliations

Umbilical cord as an analytical matrix - A technical note

Authors

Affiliations

Abstract

Conflict of interest statement

Similar articles

Cited by

Publication types

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources