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. 2020 Jan;2(1):97-109.
doi: 10.1038/s42255-019-0152-6. Epub 2019 Dec 23.

Single-cell Analysis of Human Adipose Tissue Identifies Depot and Disease Specific Cell Types

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Single-cell Analysis of Human Adipose Tissue Identifies Depot and Disease Specific Cell Types

Jinchu Vijay et al. Nat Metab. .

Abstract

The complex relationship between metabolic disease risk and body fat distribution in humans involves cellular characteristics which are specific to body fat compartments. Here we show depot-specific differences in the stromal vascual fraction of visceral and subcutaneous adipose tissue by performing single-cell RNA sequencing of tissue specimen from obese individuals. We characterize multiple immune cells, endothelial cells, fibroblasts, adipose and hematopoietic stem cell progenitors. Subpopulations of adipose-resident immune cells are metabolically active and associated with metabolic disease status and those include a population of potential dysfunctional CD8+ T cells expressing metallothioneins. We identify multiple types of adipocyte progenitors that are common across depots, including a subtype enriched in individuals with type 2 diabetes. Depot-specific analysis reveals a class of adipocyte progenitors unique to visceral adipose tissue, which shares common features with beige preadipocytes. Our human single-cell transcriptome atlas across fat depots provides a resource to dissect functional genomics of metabolic disease.

Conflict of interest statement

Declaration of Interests A.T. receives funding from Johnson & Johnson Medical Companies and Medtronic for research unrelated to the present manuscript.

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