Methanol production by polymer-encapsulated methanotrophs from simulated biogas in the presence of methane vector

Abstract

Type I (Methylomicrobium album) and II (Methyloferula stellata) methanotrophs were encapsulated by alginate and polyvinyl alcohol (PVA) to improve methanol production from simulated biogas [methane (CH₄) and carbon dioxide (CO₂)] in the presence of CH₄ vector. Polymeric matrix alginate (2%) and PVA (10%) were found to be optimum for the immobilization of both the methanotrophs, with a relative efficiency of methanol production up to 80.6 and 88.7%, respectively. The stability of methanol production by immobilized cells was improved up to 13.2-fold under repeated batch-culture over free cells. The addition of CH₄ vectors showed 1.7-fold higher methanol production on using simulated biogas than in the control. The maximum methanol production of 7.46 and 7.14 mmol/L was noted for PVA-encapsulated M. album and M. stellata, respectively. This study successfully established the beneficial effects of CH₄ vectors on methanol production by methanotrophs from greenhouse gases that can be applied for real biogas feedstock.

Keywords: Encapsulation; Greenhouse gases; Methane vector; Methanol production.