GABAergic subsensitivity of dorsal raphe neurons in vitro after chronic benzodiazepine treatment in vivo

Brain Res. 1988 Nov 8;473(1):198-202. doi: 10.1016/0006-8993(88)90336-8.


Previous studies have shown that chronic benzodiazepine treatment reduces the in vivo sensitivity of dorsal raphe neurons (DRN) to microiontophoretically applied gamma-aminobutyric acid (GABA). We have examined sensitivity of DRN in vitro using a modified midbrain slice technique which allows side-by-side analysis of slices from control and chronic diazepam-treated rats. GABA sensitivity of raphe neurons was reduced in slices from rats treated for 3 weeks with diazepam, compared to control sensitivity. Thus, GABA subsensitivity following chronic diazepam treatment appears to be dependent on changes intrinsic to the midbrain area.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Action Potentials / drug effects
  • Animals
  • Diazepam / administration & dosage*
  • In Vitro Techniques
  • Male
  • Raphe Nuclei / drug effects
  • Raphe Nuclei / metabolism
  • Raphe Nuclei / physiology*
  • Rats
  • Rats, Inbred Strains
  • gamma-Aminobutyric Acid / pharmacology*


  • gamma-Aminobutyric Acid
  • Diazepam