Nuclear scaffold attachment sites in the human globin gene complexes

EMBO J. 1988 Nov;7(11):3337-44.

Abstract

In an analysis of a 90-kb region around the human beta-globin gene complex we have identified at least eight sites of attachment to the nuclear scaffold (SARs). While these have many potential functions, there appears to be a particular association with sequences important in the regulation of the complex. Two SARs are close to the known enhancer-like elements of the beta-globin gene. SARs flanking the complex co-habit with the boundaries of the putative beta-like globin gene regulatory domain. In contrast, we have detected no SARs within a 140-kb region of the human alpha-globin gene complex. If SARs play a role in the regulation of gene expression then this structural difference would imply a difference in the regulation of the two complexes.

MeSH terms

  • Binding Sites
  • Blotting, Southern
  • Cell Nucleus / metabolism*
  • DNA / analysis*
  • DNA / genetics
  • Gene Expression Regulation
  • Globins / genetics*
  • Humans
  • Multigene Family*
  • Nucleic Acid Hybridization
  • Restriction Mapping

Substances

  • Globins
  • DNA