Identification of an Emergent Pathogen, Bartonella vinsonii, Using Next-Generation Sequencing in a Patient With Culture-Negative Endocarditis

Rachel D Downey; Susan M Russo; Sarmistha B Hauger; Donald K Murphey; Grace Marx; Thanhthao Huynh; Amy M Denison; Rachel Quirt; Ann Bailey; Marisol Fernandez

doi:10.1093/jpids/piaa014

Identification of an Emergent Pathogen, Bartonella vinsonii, Using Next-Generation Sequencing in a Patient With Culture-Negative Endocarditis

J Pediatric Infect Dis Soc. 2021 Mar 26;10(2):213-216. doi: 10.1093/jpids/piaa014.

Authors

Rachel D Downey¹, Susan M Russo¹, Sarmistha B Hauger^{1

2}, Donald K Murphey^{1

2}, Grace Marx³, Thanhthao Huynh⁴, Amy M Denison⁴, Rachel Quirt¹, Ann Bailey¹, Marisol Fernandez^{1

2}

Affiliations

¹ Dell Children's Medical Center, Austin, Texas, USA.
² Dell Medical School, University of Texas at Austin, Austin, Texas, USA.
³ Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.
⁴ Infectious Diseases Pathology Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

PMID: 32092135
DOI: 10.1093/jpids/piaa014

Abstract

Diagnosis and treatment of culture negative endocarditis remains a challenge. This report describes a rare cause of endocarditis in humans, Bartonella vinsonii, identified through next generation sequencing of plasma microbial cell-free DNA with confirmation of cardiac valve tissue infection through immunohistochemical staining and polymerase chain reaction.

Keywords: Bartonella vinsonii; diagnostics; endocarditis; glomerulonephritis; next-generation sequencing.

© The Author(s) 2020. Published by Oxford University Press on behalf of The Journal of the Pediatric Infectious Diseases Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

MeSH terms

Bartonella
Bartonella Infections* / diagnosis
Bartonella Infections* / drug therapy
Endocarditis*
Endocarditis, Bacterial* / diagnosis
Endocarditis, Bacterial* / drug therapy
High-Throughput Nucleotide Sequencing
Humans

Supplementary concepts

Bartonella vinsonii