Qualitative studies on alpha-interferon-2b in prolonged continuous infusion regimes using gradient elution high-performance liquid chromatography

J Clin Pharm Ther. 1988 Jun;13(3):225-31. doi: 10.1111/j.1365-2710.1988.tb00185.x.


The treatment of home-based cancer patients with continuous infusion chemotherapy requires that complete courses of medication in pre-filled syringes are provided on an out-patient basis. The infusion must remain stable prior to use during refrigerated storage (up to 14 days) and during infusion from holster-worn ambulatory infusion pumps where the temperature of the infusion can reach 37 degrees C. In this study, polypropylene syringes containing interferon alpha-2b infusion (3 mega units in 6 ml) were stored at 4 degrees C. The infusion was analysed during storage by a qualitative gradient-elution high-performance liquid chromatography procedure. Over 14 days storage at 4 degrees C or 24 h storage at 37 degrees C, chromatographic changes occurred indicating interconversion between interferon monomers and possibly oligomer formation. Until further studies are completed we consider interferon alpha-2b unsuitable for inclusion in our home oncology programme.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Drug Stability
  • Humans
  • Infusions, Parenteral
  • Interferon Type I* / administration & dosage
  • Interferon Type I* / analysis
  • Refrigeration
  • Syringes
  • Time Factors


  • Interferon Type I