Next generation sequencing of BRCA genes in glioblastoma multiform Egyptian patients: a pilot study

Arch Physiol Biochem. 2022 Jun;128(3):809-817. doi: 10.1080/13813455.2020.1729814. Epub 2020 Feb 26.


Background: Germ line mutations of BRCA1 and BRCA2 were correlated with a variety of cancer Authors aimed to use next-generation sequencing (NGS) to detect BRCA1 and BRCA2 germ line mutations in glioblastoma multiform (GBM) Egyptian patients.

Materials and methods: Genomic DNA was extracted from six GBM cases, amplified using Ion AmpliSeq BRCA1 and BRCA2 panel. DNA libraries were pooled, barcoded and finally sequenced using Ion Torrent Personal Genome Machine sequencer.

Results: BRCA1 the previously reported rs1799966, rs1799950, rs16941 were found in five cases and they are in a linkage disequilibrium forming two distinct haplotypes, which might support their role in cancer predisposition. Out of the 18 reported variants in BRCA2, three denovo mutations were detected which leads to frame shift.

Conclusion: Further studies on large number of GBM patients and control cases to determine BRCA1 and BRCA2 germline mutations and haplotypes; diagnostic and prognostic role are encouraged.

Keywords: BRCA genes; Glioblastoma multiform; NGS; mutations; sequencing; tumour suppressor.

MeSH terms

  • Egypt
  • Female
  • Genes, BRCA1*
  • Genes, BRCA2*
  • Genetic Predisposition to Disease
  • Glioblastoma* / genetics
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Pilot Projects