Blautia faecicola sp. nov., isolated from faeces from a healthy human

Abstract

An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111^T, was isolated from faeces from a healthy Korean. Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111^T was closely related to Ruminococcus gauveauii CCRI-16110^T (93.9 %) and Blautia stercoris GAM6-1^T (93.7 %), followed by Clostridium nexile DSM 1787^T (93.5 %), Blautia producta ATCC 27340^T (93.4 %), Blautia hydrogenotrophica DSM 10507^T (93.1 %) and Blautia coccoides ATCC 29236^T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111^T formed a separate branch with species in the genus Blautia. The major cellular fatty acids (>10.0 %) were C_{16 : 0} and C_{18 : 1} cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids. KGMB01111^T contained meso-diaminopimelic acid in cell-wall peptidoglycan. The predominant end product of fermentation produced by KGMB01111^T was acetic acid. Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111^T represents a novel species within the genus Blautia for which the name Blautia faecicola sp. nov. is proposed. The type strain is KGMB01111^T (=KCTC 15706^T=DSM 107827^T).

Keywords: Blautia faecicola sp. nov.; human faeces; microbiome; taxonomy.