Mass cytometry reveals cellular fingerprint associated with IgE+ peanut tolerance and allergy in early life

Nat Commun. 2020 Feb 27;11(1):1091. doi: 10.1038/s41467-020-14919-4.


IgE-mediated peanut allergic is common, often serious, and usually lifelong. Not all individuals who produce peanut-specific IgE will react upon consumption of peanut and can eat the food without adverse reactions, known as sensitized tolerance. Here, we employ high-dimensional mass cytometry to define the circulating immune cell signatures associated with sensitized tolerance and clinical allergy to peanut in the first year of life. Key features of clinical peanut allergic are increased frequency of activated B cells (CD19hiHLADRhi), overproduction of TNFα and increased frequency of peanut-specific memory CD4 T cells. Infants with sensitized tolerance display reduced frequency but hyper-responsive naive CD4 T cells and an increased frequency of plasmacytoid dendritic cells. This work demonstrates the utility and power of high-dimensional mass cytometry analysis to interrogate the cellular interactions that are associated with allergic sensitization and clinical food allergy in the first year of life.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / immunology*
  • Arachis / immunology*
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • Cells, Cultured
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism
  • Female
  • Humans
  • Immune Tolerance / immunology*
  • Immunoglobulin E / immunology*
  • Immunoglobulin E / metabolism
  • Immunologic Memory
  • Infant
  • Male
  • Mass Spectrometry / methods
  • Peanut Hypersensitivity / diagnosis
  • Peanut Hypersensitivity / immunology*
  • Primary Cell Culture
  • Tumor Necrosis Factor-alpha / immunology
  • Tumor Necrosis Factor-alpha / metabolism


  • Allergens
  • TNF protein, human
  • Tumor Necrosis Factor-alpha
  • Immunoglobulin E