Development of Epitope-Based Peptide Vaccine Against Novel Coronavirus 2019 (SARS-COV-2): Immunoinformatics Approach

J Med Virol. 2020 Jun;92(6):618-631. doi: 10.1002/jmv.25736. Epub 2020 Mar 5.

Abstract

Recently, a novel coronavirus (SARS-COV-2) emerged which is responsible for the recent outbreak in Wuhan, China. Genetically, it is closely related to SARS-CoV and MERS-CoV. The situation is getting worse and worse, therefore, there is an urgent need for designing a suitable peptide vaccine component against the SARS-COV-2. Here, we characterized spike glycoprotein to obtain immunogenic epitopes. Next, we chose 13 Major Histocompatibility Complex-(MHC) I and 3 MHC-II epitopes, having antigenic properties. These epitopes are usually linked to specific linkers to build vaccine components and molecularly dock on toll-like receptor-5 to get binding affinity. Therefore, to provide a fast immunogenic profile of these epitopes, we performed immunoinformatics analysis so that the rapid development of the vaccine might bring this disastrous situation to the end earlier.

Keywords: SARS-COV-2; epitopes; immunoinformatics; vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Betacoronavirus / genetics
  • Betacoronavirus / immunology*
  • Betacoronavirus / pathogenicity
  • Binding Sites
  • Computational Biology / methods
  • Coronavirus Infections / immunology
  • Coronavirus Infections / prevention & control*
  • Coronavirus Infections / virology
  • Epitopes / chemistry
  • Epitopes / genetics
  • Epitopes / immunology
  • Epitopes, B-Lymphocyte / chemistry*
  • Epitopes, B-Lymphocyte / genetics
  • Epitopes, B-Lymphocyte / immunology
  • Epitopes, T-Lymphocyte / chemistry*
  • Epitopes, T-Lymphocyte / genetics
  • Epitopes, T-Lymphocyte / immunology
  • Histocompatibility Antigens Class I / chemistry
  • Histocompatibility Antigens Class I / genetics
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class II / chemistry
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / immunology
  • Humans
  • Middle East Respiratory Syndrome Coronavirus / genetics
  • Middle East Respiratory Syndrome Coronavirus / immunology
  • Middle East Respiratory Syndrome Coronavirus / pathogenicity
  • Molecular Docking Simulation
  • Pandemics / prevention & control*
  • Pneumonia, Viral / immunology
  • Pneumonia, Viral / prevention & control*
  • Pneumonia, Viral / virology
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Protein Structure, Tertiary
  • SARS Virus / genetics
  • SARS Virus / immunology
  • SARS Virus / pathogenicity
  • Spike Glycoprotein, Coronavirus / chemistry*
  • Spike Glycoprotein, Coronavirus / genetics
  • Spike Glycoprotein, Coronavirus / immunology
  • Toll-Like Receptor 5 / chemistry*
  • Toll-Like Receptor 5 / genetics
  • Toll-Like Receptor 5 / immunology
  • Vaccines, Subunit
  • Viral Vaccines / chemistry*
  • Viral Vaccines / immunology

Substances

  • COVID-19 vaccine
  • Epitopes
  • Epitopes, B-Lymphocyte
  • Epitopes, T-Lymphocyte
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Spike Glycoprotein, Coronavirus
  • TLR5 protein, human
  • Toll-Like Receptor 5
  • Vaccines, Subunit
  • Viral Vaccines
  • spike protein, SARS-CoV-2

Supplementary concepts

  • COVID-19
  • severe acute respiratory syndrome coronavirus 2