Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis

Shixiu Cui; Hongzhi Xia; Taichi Chen; Yang Gu; Xueqin Lv; Yanfeng Liu; Jianghua Li; Guocheng Du; Long Liu

doi:10.1016/j.isci.2020.100918

Cell Membrane and Electron Transfer Engineering for Improved Synthesis of Menaquinone-7 in Bacillus subtilis

iScience. 2020 Mar 27;23(3):100918. doi: 10.1016/j.isci.2020.100918. Epub 2020 Feb 14.

Authors

Shixiu Cui¹, Hongzhi Xia², Taichi Chen¹, Yang Gu¹, Xueqin Lv¹, Yanfeng Liu¹, Jianghua Li¹, Guocheng Du¹, Long Liu³

Affiliations

¹ Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
² Richen Bioengineering Co., Ltd, Nantong 226000, China.
³ Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address: longliu@jiangnan.edu.cn.

Abstract

The formation of biofilm facilitates the synthesis of valuable natural product menaquinone-7 (MK-7) in static culture of Bacillus subtilis, whereas the essential role and mechanism of biofilm in MK-7 synthesis have not been revealed. Herein, comparative transcriptomics show that the formation of biofilm affected MK-7 synthesis by changing the transcription levels of signal receptor (BSU02010), transmembrane transporter (BSU29340, BSU03070), and signal transduction (BSU02630). Moreover, we also found that oxalate decarboxylase OxdC has an important effect on electron generation and MK-7 synthesis, when the transcriptional level of NADH dehydrogenase decreases in static culture. Our results revealed that cell membrane and electron transfer are important factors in promoting MK-7 synthesis.

Keywords: Biotechnology; Metabolic Engineering; Microbial Biotechnology.