Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations

Tej B Shrestha; Matthew T Basel

doi:10.1007/978-1-0716-0364-2_2

Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations

Methods Mol Biol. 2020:2126:13-20. doi: 10.1007/978-1-0716-0364-2_2.

Authors

Tej B Shrestha^{1

2}, Matthew T Basel³

Affiliations

¹ Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA. tbs3@vet.k-state.edu.
² Nanotechnology Innovation Center of Kansas State, Kansas State University, Manhattan, KS, USA. tbs3@vet.k-state.edu.
³ Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.

PMID: 32112375
DOI: 10.1007/978-1-0716-0364-2_2

Abstract

Luminol-based bioluminescence imaging allows noninvasive tracking of oxidatively active cells such as neutrophils. Luminol is given intravenously or intraperitoneally, followed by bioluminescence imaging at 425 nm. Here we describe a method for tracking neutrophil extravasation into an inflammatory site, especially focusing on mammary carcinoma.

Keywords: Bioluminescence; Luminol; Myeloperoxidase; NADPH oxidase; Neutrophil.

MeSH terms

Animals
Cell Tracking / methods*
Female
Inflammation / immunology*
Inflammation / pathology
Luminescent Measurements / methods*
Luminol / metabolism*
Mammary Neoplasms, Animal / immunology
Mammary Neoplasms, Animal / metabolism
Mammary Neoplasms, Animal / pathology*
Mice
Mice, Inbred BALB C
NADPH Oxidases / metabolism*
Neutrophils / immunology*
Neutrophils / pathology
Peroxidase / metabolism*

Substances

Luminol
Peroxidase
NADPH Oxidases