The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells

J Neuroimmunol. 2020 Apr 15;341:577191. doi: 10.1016/j.jneuroim.2020.577191. Epub 2020 Feb 13.


Morphine- and Concanavalin A-induced changes of protein composition of rat spleen lymphocytes were determined by high-resolution proteomic analysis, gel-free, label-free quantification, MaxLFQ. Stimulation by Con A resulted in a major reorganization of spleen cell protein composition evidenced by increased expression level of 94 proteins; 101 proteins were down-regulated (>2-fold). Interestingly, among proteins that were up-regulated to the largest extent were the prototypical brain proteins as a neuron specific enolase, synapsin-1, brain acid-soluble protein-1 and myelin basic protein. Morphine-induced change was limited to no more than 5 up-regulated and 18 down-regulated proteins (>2-fold).

Keywords: Concanavalin A; Morphine; Proteomic analysis; Rat spleen lymphocytes; Up-regulation of brain proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Concanavalin A / pharmacology*
  • Gene Expression Profiling
  • Gene Expression Regulation / drug effects*
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / genetics
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism
  • Male
  • Morphine / pharmacology*
  • Proteome / drug effects*
  • Proteomics / methods*
  • Rats
  • Rats, Wistar
  • Spleen / cytology*


  • Proteome
  • Concanavalin A
  • Morphine