The protective effect of betanin and copper on heart and lung in end‑organ ischemia reperfusion injury

Bratisl Lek Listy. 2020;121(3):211-217. doi: 10.4149/BLL_2020_032.


Objectives: Betanin and copper sulphate have been previously indicated as beneficial agents for ischemia/reperfusion (I/R) as antioxidant compounds in various models. We investigated whether betanin and copper have any protective effects on the heart and lung against I/R injury in rats.

Methods: Spraque-Dawley rats were assigned in groups: Sham (laparotomy only), control (I/R only), betanin treatment (100 mg/kg of betanin administered intraperitoneally (i.p.) 60 minutes before I/R) and copper sulfate treatment group (0.1 mg/kg/day copper sulfate i.p. for 7 days before I/R). Ischemia was induced by clamping the aorta between the left renal artery and aortic bifurcation for 45 minutes. After 48-hour reperfusion, the rats were sacrificed and heart/lung tissues were harvested. Malondialdehyde (MDA), myeloperoxidase (MPO), interleukin 6 (IL-6) levels were determined. Apoptosis was determined via TUNEL assay.

Results: MDA, MPO, IL-6 levels and apoptotic cells were significantly increased in the I/R group. In both treatment groups, MDA and MPO levels were decreased. IL-6 was significantly decreased in response to betanin administration in the heart, but not in the lung; copper had no effect in either area. The numbers of apoptotic cells were significantly decreased in both treatment groups.

Conclusion: Betanin and copper may have protective effects on I/R injury in the heart and lung in rats (Fig. 6, Ref. 39).

Keywords: antiinflammation; antioxidant rat.; betanin; copper; ischemia reperfusion injury.

MeSH terms

  • Animals
  • Betacyanins* / pharmacology
  • Copper* / pharmacology
  • Heart / drug effects
  • Lung / drug effects
  • Malondialdehyde
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury* / prevention & control


  • Betacyanins
  • Malondialdehyde
  • Copper