Proteomics Computational Analyses Suggest That the Envelope Glycoproteins of Segmented Jingmen Flavi-Like Viruses are Class II Viral Fusion Proteins (b-Penetrenes) with Mucin-Like Domains

Viruses. 2020 Feb 27;12(3):260. doi: 10.3390/v12030260.


Jingmen viruses are newly described segmented flavi-like viruses that have a worldwide distribution in ticks and have been associated with febrile illnesses in humans. Computational analyses were used to predict that Jingmen flavi-like virus glycoproteins have structural features of class II viral fusion proteins, including an ectodomain consisting of beta-sheets and short alpha-helices, a fusion peptide with interfacial hydrophobicity and a three-domain architecture. Jingmen flavi-like virus glycoproteins have a sequence enriched in serine, threonine, and proline at the amino terminus, which is a feature of mucin-like domains. Several of the serines and threonines are predicted be modified by the addition of O-linked glycans. Some of the glycoproteins are predicted to have an additional mucin-like domain located prior to the transmembrane anchor, whereas others are predicted to have a stem consisting of two alpha-helices. The flavivirus envelope protein and Jingmen flavi-virus glycoproteins may have diverged from a common class II precursor glycoprotein with a mucin-like domain or domains acquired after divergence.

Keywords: Alongshan virus; Jingmen tick virus; O-liked glycans; class II viral fusion proteins; flavi-like viruses; mucin-like domain; virus evolution.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Computational Biology / methods
  • Flavivirus / genetics*
  • Flavivirus / metabolism*
  • Genome, Viral
  • Genomics / methods
  • Humans
  • Models, Molecular
  • Molecular Conformation
  • Mucins / chemistry
  • Mucins / metabolism
  • Protein Interaction Domains and Motifs*
  • Proteome*
  • Proteomics* / methods
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / metabolism*
  • Viral Fusion Proteins / chemistry
  • Viral Fusion Proteins / metabolism*


  • Mucins
  • Proteome
  • Viral Envelope Proteins
  • Viral Fusion Proteins