Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L

Jinyan Gu; Haihui Zhang; Hui Yao; Jie Zhou; Yuqing Duan; Haile Ma

doi:10.1016/j.carbpol.2020.115939

Comparison of characterization, antioxidant and immunological activities of three polysaccharides from Sagittaria sagittifolia L

Carbohydr Polym. 2020 May 1:235:115939. doi: 10.1016/j.carbpol.2020.115939. Epub 2020 Feb 4.

Authors

Jinyan Gu¹, Haihui Zhang², Hui Yao¹, Jie Zhou¹, Yuqing Duan³, Haile Ma⁴

Affiliations

¹ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China.
² School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang, 212013, China. Electronic address: zhanghh@ujs.edu.cn.
³ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang, 212013, China. Electronic address: dyq101@ujs.edu.cn.
⁴ School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang, 212013, China.

PMID: 32122481
DOI: 10.1016/j.carbpol.2020.115939

Abstract

To investigate and compare the preliminary structural characteristics and biological activity in vitro of polysaccharides from Sagittaria sagittifolia L. (SSs) by different extration methods, three polysaccharides (SSW, SSU, and SSP) were obtained with hot water, ultrasound-assisted, and subcritical water extraction. Their structural features were elucidated using High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Scanning Electron Microscopy (SEM), Infrared Spectroscopy (IR), Atomic Force Microscopy (AFM), Zeta Potential and Congo red methods. Furthermore, the antioxidant activity and immunostimulatory effects were investigated in vitro. Molecular weight and monosaccharide composition analysis exhibited that SSW (2275.0 kDa), SSU (148.7 kDa), and SSP (1984.0 kDa) were heteropolysaccharide with dramatically different monosaccharide species and mole ratios. In addition, SSP exhibited stronger antioxidant activity in vitro and more potent immunomodulatory activity than SSW and SSU. SSP has greater potential to be explored as biologicalagents for use in complementary medicine or functional foods.

Keywords: Antioxidant and immunomodulatory activity; Preliminary structure; Sagittaria sagittifolia L. polysaccharides; Subcritical water extraction; Ultrasound-assisted extraction.

Publication types

Comparative Study

MeSH terms

Animals
Antioxidants / chemistry
Antioxidants / pharmacology*
Benzothiazoles / antagonists & inhibitors
Biphenyl Compounds / antagonists & inhibitors
Cell Proliferation / drug effects
Cell Survival / drug effects
Cell Survival / immunology
Cells, Cultured
Hydroxyl Radical / antagonists & inhibitors
Immunologic Factors / chemistry
Immunologic Factors / pharmacology*
Macrophages / drug effects
Macrophages / immunology
Mice
Molecular Weight
Nitric Oxide / analysis
Nitric Oxide / biosynthesis
Particle Size
Picrates / antagonists & inhibitors
Polysaccharides / chemistry
Polysaccharides / pharmacology*
RAW 264.7 Cells
Sagittaria / chemistry*
Sulfonic Acids / antagonists & inhibitors
Surface Properties

Substances

Antioxidants
Benzothiazoles
Biphenyl Compounds
Immunologic Factors
Picrates
Polysaccharides
Sulfonic Acids
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
Nitric Oxide
Hydroxyl Radical
1,1-diphenyl-2-picrylhydrazyl