Skewed distribution of leaf color RGB model and application of skewed parameters in leaf color description model

Plant Methods. 2020 Feb 26:16:23. doi: 10.1186/s13007-020-0561-2. eCollection 2020.

Abstract

Background: Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters and limited applicability.

Results: We confirmed the skewness distribution characteristics of the red, green, blue and grayscale channels of the images of tobacco leaves. Twenty skewed-distribution parameters were computed including the mean, median, mode, skewness, and kurtosis. We used the mean parameter to establish a stepwise regression model that is similar to earlier models. Other models based on the median and the skewness parameters led to accurate RGB-based description and prediction, as well as better fitting of the SPAD value. More parameters improved the accuracy of RGB model description and prediction, and extended its application range. Indeed, the skewed-distribution parameters can describe changes of the leaf color depth and homogeneity.

Conclusions: The color histogram of the blade images follows a skewed distribution, whose parameters greatly enrich the RGB model and can describe changes in leaf color depth and homogeneity.

Keywords: Leaf color; RGB model; SPAD; Skewed distribution; Skewed parameters.