A microarray platform designed for high-throughput screening the reaction conditions for the synthesis of micro/nanosized biomedical materials

Xiaoyu Li; Xiran Yang; Lei Liu; Peipei Zhou; Jianhua Zhou; Xuetao Shi; Yingjun Wang

doi:10.1016/j.bioactmat.2020.02.003

A microarray platform designed for high-throughput screening the reaction conditions for the synthesis of micro/nanosized biomedical materials

Bioact Mater. 2020 Feb 25;5(2):286-296. doi: 10.1016/j.bioactmat.2020.02.003. eCollection 2020 Jun.

Authors

Xiaoyu Li^{1

2

3

4

5}, Xiran Yang^{1

2

3

4

5}, Lei Liu^{1

2

3

4

5}, Peipei Zhou⁶, Jianhua Zhou⁶, Xuetao Shi^{1

2

3

4

5}, Yingjun Wang^{1

2

3

4

5}

Affiliations

¹ School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China.
² National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China.
³ Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou, 510006, PR China.
⁴ Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China.
⁵ Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China.
⁶ School of Engineering, Sun Yat-sen University, Guangzhou, 510275, PR China.

Abstract

Materials research usually relies on lengthy and largely trial-and-error methods, high-throughput technology has thereby emerged as an alternative method which is proven to be a simple, rapid, accurate and sensitive technique. Here, we presented a microfluidic platform with a set of 6 × 6 microarray chips for high-throughput synthesis and rapid screening the reaction conditions of biomedical materials. The core design of this platform is to generate concentration gradient inside microarray chips. Considering that calcium phosphates (CaP) are the most important inorganic constituents of biological hard tissues, different phases of calcium phosphates particles were synthesized with various morphogenesis when the reaction conditions such as Ca/P concentration ratio, NaOH concentration were screened using our platform. And this platform is universal and expected to apply to other systems for high-throughput screening and synthesis.

Keywords: Calcium phosphates; Concentration gradient; High-throughput; Microfluidics.