Induction of secondary metabolite production by hygromycin B and identification of the 1233A biosynthetic gene cluster with a self-resistance gene

J Antibiot (Tokyo). 2020 Jul;73(7):475-479. doi: 10.1038/s41429-020-0295-4. Epub 2020 Mar 5.

Abstract

We found that the protein synthesis inhibitor hygromycin B induced the production of secondary metabolites, including lucilactaene, NG-391, fusarubin, 1233A, and 1233B, in the filamentous fungus, Fusarium sp. RK97-94. We identified the biosynthetic gene cluster for 1233A, an HMG-CoA synthase inhibitor. The biosynthetic gene cluster consisted of four genes, one of which was involved in conferring self-resistance to 1233A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fatty Acids, Unsaturated / genetics*
  • Fungi / genetics
  • Fungi / metabolism
  • Furans / metabolism
  • Fusarium / genetics
  • Fusarium / metabolism
  • Hydroxymethylglutaryl-CoA Synthase / antagonists & inhibitors
  • Hygromycin B / metabolism*
  • Lactones
  • Multigene Family / genetics*
  • Naphthoquinones / metabolism
  • Pyrroles / metabolism

Substances

  • Fatty Acids, Unsaturated
  • Furans
  • Lactones
  • Naphthoquinones
  • Pyrroles
  • fusarubin
  • lucilactaene
  • antibiotic 1233A
  • Hygromycin B
  • Hydroxymethylglutaryl-CoA Synthase