Genome-wide identification and characterization of the BES1 gene family in apple (Malus domestica)

Abstract

As the most important transcription factor in the brassinosteroid (BR) signal transduction pathway, BES1 not only affects growth and development of plants but also regulates stress resistance of crops. The physicochemical properties, gene structure, cis-acting elements and gene chip expression of apple BES1 transcription factors were analysed using bioinformatics, and expression of this gene family was analysed with qRT-PCR. There were 22 members of the apple BES1 transcription factors, distributed on eight chromosomes, divided into seven subtribes (I-VII), and the same subtribe contained the same basic motifs. Gene structure analysis showed that the number and position of exons differed, and there was no upstream and downstream structure. Analysis of cis-acting elements indicated that BES1 transcription factors contain response elements for hormones and abiotic stress, as well as organ-specific elements. Gene chip expression profile analysis revealed that expression patterns of BES1 transcription factors differed in different apple hybrids and different organs. In addition, expression of apple BES1 genes was higher in flowers, young fruits, mature fruits and leaves. qRT-PCR demonstrated that expression of MdBES1 genes was highest 12 h after BR induction. At the same time, there were differences in expression in response to PEG, NaCl and MeJA. This paper provides a theoretical basis for analysis of the biological function and stress resistance mechanism of BES1 transcription factors in apple.

Keywords: Apple; BES1 transcription factors; Cis-acting elements; bioinformatics; chip expression profiling; qRT-PCR.