A pilot study: VereMTB detection kit for rapid detection of multidrug-resistant mycobcterium tuberculosis in clinical sputum samples

PLoS One. 2020 Mar 9;15(3):e0228312. doi: 10.1371/journal.pone.0228312. eCollection 2020.

Abstract

The performance of VereMTBTM Detection Kit for detection of multidrug-resistant tuberculosis (MDR-TB) was validated by using 124 sputum samples. Comparing with MGIT culture, the sensitivity and specificity of VereMTB Detection Kit for MTBC detection were 97.0% and 98.3%, respectively. Compared with MGIT DST, the sensitivity and specificity of VereMTB Detection Kit for RIF resistance detection were 85.7% and 93.9%, respectively, and the sensitivity and specificity of VereMTB Detection Kit for INH resistance detection were 75.0% and 95.7%, respectively. 6 NTM samples were also detected and identified correctly. The VereMTB Detection Kit can detect MDR-TB rapidly and accurately in sputum samples from TB suspects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitubercular Agents / pharmacology
  • Drug Resistance, Multiple*
  • Humans
  • Isoniazid / pharmacology
  • Limit of Detection
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / isolation & purification*
  • Pilot Projects
  • Rifampin / pharmacology
  • Sputum / microbiology*
  • Time Factors

Substances

  • Antitubercular Agents
  • Isoniazid
  • Rifampin

Grants and funding

Funding was provided by the National Science and Technology Major Project (2018ZX10103001) and the National Natural Science Foundation of China (8161101571). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.