Magnetic Resonance Imaging Property of Doxorubicin-Loaded Gadolinium/13X Zeolite/Folic Acid Nanocomposite

Ghaderi S; Divband B; Gharehaghaji N

doi:10.31661/jbpe.v0i0.1254

Magnetic Resonance Imaging Property of Doxorubicin-Loaded Gadolinium/13X Zeolite/Folic Acid Nanocomposite

J Biomed Phys Eng. 2020 Feb 1;10(1):103-110. doi: 10.31661/jbpe.v0i0.1254. eCollection 2020 Feb.

Authors

Ghaderi S^{1

2}, Divband B^{3

4}, Gharehaghaji N⁵

Affiliations

¹ MSc, Medical Radiation Sciences Research Team, Tabriz University of Medical Sciences, Tabriz, Iran.
² MSc, Department of Radiology, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
³ PhD, Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁴ PhD, Inorganic Chemistry Department, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
⁵ PhD, Department of Radiology, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Background: Magnetic resonance imaging (MRI) using nanostructures has been a proper method for tumor targeting purposes. Different MRI nanomaterials, targeting agents and anticancer drugs have been used for targeting of tumors.

Objectives: This study aims to consider the MRI property of doxorubicin (DOX)-loaded gadolinium/13X zeolite/folic acid (Gd³⁺/13X/FA) nanocomposite.

Material and methods: In this in vitro study, Gd³⁺/13X/FA/DOX nanocomposite was prepared and the X-ray diffraction, scanning electron microscopy and MTT assay were conducted to evaluate the physicochemical properties of the nanocomposite. MRI was performed at 25°C using a 1.5 T clinical system to determine the T1 relaxation times and subsequently, the T1 relaxivity.

Results: The size of the nanocomposite was in the range of 80-200 nm. The nanocomposite without DOX loading (Gd³⁺/13X/FA) showed compatibility for A549 cells for all concentrations while DOX-loaded nanocomposite was toxic for 62% of the cells at the concentration of 0.4 mg/ml. The T1 relaxivity of Gd³⁺/13X/FA/DOX nanocomposite was 4.0401 mM^-1s^-1.

Conclusion: Gd³⁺/13X/FA/DOX nanocomposite shows a T1 relaxivity similar to the conventional gadolinium chelates, and a successful DOX loading.

Keywords: 13X zeolite; Doxorubicin; Folic Acid; Gadolinium; Magnetic Resonance Imaging; Relaxivity.