Abstract
We detail a heterobifunctional, 7-aminocoumarin photocleavable (PC) linker with unique properties to covalently attach Abs to surfaces and subsequently release them with visible light (400-450 nm). The PC linker allowed rapid (2 min) and efficient (>90%) release of CTCs and EVs without damaging their molecular cargo.
MeSH terms
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Antibodies, Monoclonal / chemistry*
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Antibodies, Monoclonal / radiation effects
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Cell Line, Tumor
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Cell Survival
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Coumarins / chemistry*
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Coumarins / radiation effects
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Extracellular Vesicles*
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Humans
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Light
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Liquid Biopsy
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Microfluidics
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Neoplastic Cells, Circulating*
Substances
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Antibodies, Monoclonal
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Coumarins