A Review on the Current Methods of Chinese Hamster Ovary (CHO) Cells Cultivation for the Production of Therapeutic Protein

Curr Drug Discov Technol. 2021;18(3):354-364. doi: 10.2174/1570163817666200312102137.


Most of the clinical approved protein-based drugs or under clinical trials have a profound impact on the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of the therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to enhance specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will focus on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

Keywords: CHO cells; Therapeutic protein; bio-processing; mammalian cells; mass-production.; protein expression.

Publication types

  • Review

MeSH terms

  • Animals
  • Antibodies, Monoclonal / isolation & purification*
  • Antibodies, Monoclonal / therapeutic use
  • Bioreactors
  • CHO Cells
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods*
  • Cricetulus
  • Humans
  • Recombinant Proteins / isolation & purification*
  • Recombinant Proteins / therapeutic use
  • Technology, Pharmaceutical / instrumentation
  • Technology, Pharmaceutical / methods*


  • Antibodies, Monoclonal
  • Recombinant Proteins