Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine

Methods Mol Biol. 2020:2123:321-333. doi: 10.1007/978-1-0716-0346-8_23.


The serum virus neutralization (SVN) assay is a serological test used to detect the presence and magnitude of functional systemic antibodies that prevent infectivity of a virus. The SVN assay is a highly sensitive and specific test that may be applied to influenza A viruses (IAV) in swine to measure the titer of neutralizing antibodies postexposure, postvaccination, or after passive transfer of maternally derived antibody (MDA). Conventional SVN methods performed in vitro are based on inhibition of virus infectivity in cell culture in the presence of neutralizing antibodies in serum. Titer determination may be based on the presence or absence of cytopathic effect or evidence of viral infection using an immunoreactive technique. The SVN assay is relatively inexpensive using standard laboratory equipment, although it requires cell culture, more time and labor, and technical skill to conduct the assay compared to other serological methods. The SVN test may be used to evaluate the level of serological cross-reactivity between IAV exposure or vaccine antisera and heterologous influenza viruses that may correlate with cross-protection in the host.

Keywords: Antibody; Influenza; Serum; Swine; Titer; Virus neutralization.

MeSH terms

  • Animals
  • Antibodies, Neutralizing / blood*
  • Antibodies, Neutralizing / isolation & purification*
  • Dogs
  • Immunohistochemistry
  • Influenza A virus / immunology*
  • Madin Darby Canine Kidney Cells
  • Neutralization Tests / methods*
  • Swine / virology*


  • Antibodies, Neutralizing