Novel strategy of incorporating biochar in solid-state fermentation for enhancing erythritol production by forming "microzones"

Abstract

Biochar is increasingly considered in addressing bioprocess issues due to its strong adsorbability and excellent compatibility to microbes. Here, biochar was first applied in aerobic solid-state fermentation (SSF) for erythritol production. Biochars derived from different agricultural wastes under various pyrolysis temperatures were evaluated, and wheat straw pyrolyzed at 300 °C (WSc) performed the best in enhancing fermentative erythritol production, with a dosage of 4% (w/w). In this procedure, cell-biochar-substrate "microzones" were formed, which was conductive to cell growth and attachment, and hence contributed enhanced enzyme activities, oil consumption, and erythritol production. The resultant erythritol productions of batch and fed-batch fermentations were 207.3 and 222.5 mg/gds, respectively. In repeated-batch fermentation, high cell viability and robust erythritol synthesis were maintained throughout seven cycles. This study demonstrates that SSF can be remarkably facilitated by biochar addition, suggesting a new perspective of biochar application in microbiological processes.

Keywords: Batch fermentation; Biochar; Erythritol production; Microzones; Solid state fermentation.