Detection of mitophagy in mammalian cells, mice, and yeast

Methods Cell Biol. 2020:155:557-579. doi: 10.1016/bs.mcb.2019.10.006. Epub 2019 Nov 27.


Selective elimination of superfluous or dysfunctional mitochondria is a fundamental process conserved among both uni- and multicellular eukaryotes, contributing to mitochondrial quality and quantity control. This process depends on autophagy, a cellular self-eating membrane trafficking system, and is thus called mitophagy. In this chapter, we describe methods to detect mitophagy in mammalian cells, mice, and yeast.

Keywords: Autophagy; Lysosome; Macroautophagy; Mitochondria; Park2; Park6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cytological Techniques / methods*
  • Female
  • HeLa Cells
  • Humans
  • Lysosomes / metabolism
  • Male
  • Mice, Transgenic
  • Mitophagy*
  • Saccharomyces cerevisiae / metabolism
  • Ubiquitin / metabolism


  • Ubiquitin