Galactosylated wall teichoic acid, but not lipoteichoic acid, retains InlB on the surface of serovar 4b Listeria monocytogenes

Mol Microbiol. 2020 Mar;113(3):638-649. doi: 10.1111/mmi.14455.


Listeria monocytogenes is a Gram-positive, intracellular pathogen harboring the surface-associated virulence factor InlB, which enables entry into certain host cells. Structurally diverse wall teichoic acids (WTAs), which can also be differentially glycosylated, determine the antigenic basis of the various Listeria serovars. WTAs have many physiological functions; they can serve as receptors for bacteriophages, and provide a substrate for binding of surface proteins such as InlB. In contrast, the membrane-anchored lipoteichoic acids (LTAs) do not show significant variation and do not contribute to serovar determination. It was previously demonstrated that surface-associated InlB non-covalently adheres to both WTA and LTA, mediating its retention on the cell wall. Here, we demonstrate that in a highly virulent serovar 4b strain, two genes gtlB and gttB are responsible for galactosylation of LTA and WTA respectively. We evaluated the InlB surface retention in mutants lacking each of these two genes, and found that only galactosylated WTA is required for InlB surface presentation and function, cellular invasiveness and phage adsorption, while galactosylated LTA plays no role thereof. Our findings demonstrate that a simple pathogen-defining serovar antigen, that mediates bacteriophage susceptibility, is necessary and sufficient to sustain the function of an important virulence factor.

Keywords: Listeria monocytogenes; bacteriophages; cell wall; galactose; glycosylation; teichoic acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism*
  • Bacterial Proteins / physiology
  • Cell Wall / metabolism
  • Glycosylation
  • Lipopolysaccharides / metabolism
  • Listeria monocytogenes / metabolism*
  • Listeria monocytogenes / pathogenicity
  • Membrane Proteins / metabolism*
  • Membrane Proteins / physiology
  • Serogroup
  • Teichoic Acids / metabolism*
  • Virulence
  • Virulence Factors / metabolism


  • Bacterial Proteins
  • Lipopolysaccharides
  • Membrane Proteins
  • Teichoic Acids
  • Virulence Factors
  • inlB protein, Listeria monocytogenes
  • lipoteichoic acid