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. 2020 Mar 19.
doi: 10.1002/anie.202002417. Online ahead of print.

An Electrochemophysiological Microarray for Real-Time Monitoring and Quantification of Multiple Ions in the Brain of a Freely Moving Rat

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An Electrochemophysiological Microarray for Real-Time Monitoring and Quantification of Multiple Ions in the Brain of a Freely Moving Rat

Fan Zhao et al. Angew Chem Int Ed Engl. .

Abstract

Herein, we present an electrochemophysiological microarray (ECPM) for real-time mapping and simultaneous quantification of chemical signals for multiple ions in the deep brain of a freely moving rat, in which microelectrode arrays were developed for direct determination of multiple ions using open-circuit potentiometry. Specific recognition ionophores were synthesized and optimized for determination of K+ , Ca2+ , Na+ and pH. A reference electrode was also developed to avoid interferences in the brain. The microarrays were successfully applied in real-time monitoring and quantification of ions in a live brain. The extra current-free potentiometry allowed mapping and biosensing of chemical signals, together with recording of electrical signals in the whole brain without cross-talk, for the first time. Furthermore, the ECPM provided a platform for real-time monitoring of the dynamic changes of multiple ions in the deep brain of freely moving rat during a seizure.

Keywords: brain; electrochemistry; freely moving rats; ion channels; microelectrode arrays.

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